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Composition of sea urchin egg homogenate determines its potency to inositol trisphosphate and cyclic ADPRibose-induced Ca2+ release

Lookup NU author(s): Professor Keith Jones

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Abstract

Of the three intracellular Ca2+ signalling molecules (InsP3, cADPR and NAADP) sea urchin egg homogenate has been used in the identification and characterisation of two, cADPR and NAADP. Homogenate is prepared in a Na+/Cl- substitute of N-Methyl glucamine (NMG)/gluconate. To determine how media composition affects Ca2+ release we replaced NMG with various sugars or glycine and found a dramatic improvement in InsP3 mediated Ca2+ release. Conversely the response to cADPR was diminished, whilst NAADP was unaffected. Therefore modifying media composition may be an important consideration in using homogenate to study Ca2+ release for future studies. © 2007 Elsevier Inc. All rights reserved.


Publication metadata

Author(s): Jones KT, Swann K

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 2007

Volume: 360

Issue: 4

Pages: 815-820

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.bbrc.2007.06.134

DOI: 10.1016/j.bbrc.2007.06.134


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