Toggle Main Menu Toggle Search

Open Access padlockePrints

Effects of vasectomy surgery and meloxicam treatment on faecal corticosterone levels and behaviour in two strains of laboratory mouse

Lookup NU author(s): Dr Claire Richardson, Dr Johnny RoughanORCiD, Emeritus Professor Paul FlecknellORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Behaviour was assessed in 32 C57BL/6JCrl and 32 C3H/HeN male mice 1 h following vasectomy; saline or meloxicam was administered 30 min prior to surgery at 5, 10, or 20 mg kg-1. Faeces were collected 24 h prior to, and 3, 6, 9, 12, 24 h following, vasectomy for measurement of faecal corticosterone. Peak corticosterone levels were significantly higher in mice that underwent vasectomy and received saline (p < 0.001) or meloxicam at 5 or 10 mg kg-1 (p = 0.021, and p < 0.001, respectively) compared with normal un-operated controls. Mice that underwent vasectomy and received 20 mg kg-1 meloxicam had peak corticosterone levels that were not different from normal un-operated mice (p = 0.254). Discriminant analysis was used to identify behaviours responsible for group separation; these were summed to create two behaviour scores. Score 2 (the frequency of flinching, writhing, rear leg lift and press 2) was thought to be pain related; mice that underwent vasectomy and received saline exhibited significantly more of these behaviours than the normal controls (p = 0.032), and the mice that received meloxicam (at any dose). Strain differences were observed in both the stress response to vasectomy and the behavioural changes; the C3H/HeN mice had higher pain scores (behaviour Score 2) and peak corticosterone responses than the C57BL/6JCrl mice. We have demonstrated that significant changes occur in the behaviour of mice following vasectomy, and these changes are reduced by use of meloxicam. Vasectomy elicits a rise in corticosterone levels that was only reduced by the highest dose of meloxicam. © 2006 International Association for the Study of Pain.

Publication metadata

Author(s): Wright-Williams SL, Courade J-P, Richardson CA, Roughan JV, Flecknell PA

Publication type: Article

Publication status: Published

Journal: Pain

Year: 2007

Volume: 130

Issue: 1-2

Pages: 108-118

Print publication date: 01/07/2007

ISSN (print): 0304-3959

ISSN (electronic): 1872-6623

Publisher: Elsevier BV


DOI: 10.1016/j.pain.2006.11.003

PubMed id: 17196337


Altmetrics provided by Altmetric


Funder referenceFunder name
G0400227/1National Centre for the Replacement, Refinement and Reduction of Animals in Research