Browse by author
Lookup NU author(s): Dr Kehinde Ross,
Emeritus Professor Michael Whitaker,
Professor Nick Reynolds
The mechanisms of agonist-induced calcium entry (ACE) following depletion of intracellular calcium stores have not been fully established. We report here that calcium-independent phospholipase A (iPLA2) is required for robust Ca2+ entry in HaCaT keratinocytes following ATP or UTP stimulation. Lysophosphatidic acid (LPA), an unrelated agonist, evoked Ca 2+ release without inducing robust Ca2- entry. Both LPA and UTP induced the redistribution of STIM1 into puncta which localized to regions near or at the plasma membrane, as well as within the cytoplasm. Plasma membrane-associated STIM1 remained high for up to 10 min after UTP stimulation, whereas it had returned almost to baseline by that time point in LPA-stimulated cells. This correlated with faster reloading of the endoplasmic reticulum Ca2+ stores in LPA treated cells. Thus by differentially regulating store-refilling after agonist-mediated depletion, LPA and UTP may exert distinct effects on the duration of STIM1 localization at the plasma membrane, and thus, on the magnitude and duration of ACE. © 2007 Wiley-Liss, Inc.
Author(s): Ross K, Whitaker M, Reynolds NJ
Publication type: Article
Publication status: Published
Journal: Journal of Cellular Physiology
Print publication date: 01/06/2007
ISSN (print): 0021-9541
ISSN (electronic): 1097-4652
Publisher: John Wiley & Sons, Inc.
PubMed id: 17299780
Altmetrics provided by Altmetric