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Detection of 17O-tagged phosphate by 31P MRS: A method with potential for in vivo studies of phosphorus metabolism

Lookup NU author(s): Professor Peter Thelwall

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Abstract

We present a method for MR detection of 17O-labeled phosphate groups. The method employs the T2 relaxivity effect of 17O on 31P nuclei to distinguish between 17O-labeled and unlabeled phosphate groups, and uses spin-echo (SE) acquisitions with RF decoupling at the 17O frequency to generate 31P spectra that show only 17O-labeled phosphate groups. The method provides an alternative to spin-labeling experiments, which are limited to the study of rapidly exchanging phosphate groups by the T1 relaxation rates of phosphorus nuclei. We demonstrate separation of MR signals from labeled and unlabeled phosphate-containing compounds, and characterization of the T 2 effect of 17O on phosphate nuclei in 17O-labeled phosphate groups. Previous 17O and 18O phosphate-labeled studies used mass spectrometry or high-resolution MR spectroscopy (MRS) to detect the presence of an isotopic label, which requires ex vivo sample preparation. In our method the detection of 17O-labeled phosphate is manifested as a large change in 31P T2, and thus allows in vivo detection using simple MR methods. Thus this method may have potential for in vivo studies of bioenergetics and the metabolism of phosphate-containing compounds. © 2007 Wiley-Liss, Inc.


Publication metadata

Author(s): Thelwall PE

Publication type: Article

Publication status: Published

Journal: Magnetic Resonance in Medicine

Year: 2007

Volume: 57

Issue: 6

Pages: 1168-1172

ISSN (print): 0740-3194

ISSN (electronic): 1522-2594

Publisher: John Wiley & Sons, Inc.

URL: http://dx.doi.org/10.1002/mrm.21226

DOI: 10.1002/mrm.21226

PubMed id: 17534916


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