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Lookup NU author(s): Professor Peter Thelwall
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We present a method for MR detection of 17O-labeled phosphate groups. The method employs the T2 relaxivity effect of 17O on 31P nuclei to distinguish between 17O-labeled and unlabeled phosphate groups, and uses spin-echo (SE) acquisitions with RF decoupling at the 17O frequency to generate 31P spectra that show only 17O-labeled phosphate groups. The method provides an alternative to spin-labeling experiments, which are limited to the study of rapidly exchanging phosphate groups by the T1 relaxation rates of phosphorus nuclei. We demonstrate separation of MR signals from labeled and unlabeled phosphate-containing compounds, and characterization of the T 2 effect of 17O on phosphate nuclei in 17O-labeled phosphate groups. Previous 17O and 18O phosphate-labeled studies used mass spectrometry or high-resolution MR spectroscopy (MRS) to detect the presence of an isotopic label, which requires ex vivo sample preparation. In our method the detection of 17O-labeled phosphate is manifested as a large change in 31P T2, and thus allows in vivo detection using simple MR methods. Thus this method may have potential for in vivo studies of bioenergetics and the metabolism of phosphate-containing compounds. © 2007 Wiley-Liss, Inc.
Author(s): Thelwall PE
Publication type: Article
Publication status: Published
Journal: Magnetic Resonance in Medicine
ISSN (print): 0740-3194
ISSN (electronic): 1522-2594
Publisher: John Wiley & Sons, Inc.
PubMed id: 17534916
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