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Hyperthermia inhibits cell proliferation and induces apoptosis: Relative signaling status of P53, S100A4, and notch in heat sensitive and resistant cell lines

Lookup NU author(s): Dr Gajanan Sherbet


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The effects of hyperthermia on the expression of p53, the apoptosis-associated genes Bax and Bcl-2, Notch and S100A4 have been studied in the HepG2 cell line and the HUT cell line derived from HepG2, adapted for growth in hyperthermic conditions. Hyperthermia inhibits cell proliferation and induces apoptosis. HepG2 and HUT cells differed in respect of anchorage to growth surface, degree of proliferation and apoptosis and expression of p53, Bax, Bcl-2, Notch, and S100A4 genes. The induction of apoptosis and the inhibition of cell proliferation occurred independently of p53, and independently also of involvement of the apoptosis family genes Bax and Bcl-2. We demonstrate novel and marked differences between transient heat shock and heat adaptation in respect of pathways of signaling and generation of phenotypic effects in vitro. Different signaling patterns have been identified here. Pathways of signaling by S100A4, by its interaction with and sequestration of p53, and by Notch also seem differentially operational in the induction of apoptosis, and both appear to be activated as alternative pathways in the context of hyperthermia signaling independently of p53. © 2007 Wiley-Liss, Inc.

Publication metadata

Author(s): Basile A, Biziato D, Sherbet GV, Comi P, Cajone F

Publication type: Article

Publication status: Published

Journal: Journal of Cellular Biochemistry

Year: 2008

Volume: 103

Issue: 1

Pages: 212-220

ISSN (print): 0730-2312

ISSN (electronic): 1097-4644

Publisher: John Wiley and Sons


DOI: 10.1002/jcb.21401

PubMed id: 17497677


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