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The predictive value of p53 and p33ING1b in patients with Dukes'C colorectal cancer

Lookup NU author(s): Seamus Kelly, Dr John Anderson, Dr Brian Angus, Dr Christine Challen, Professor John Lunec

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Abstract

Objective: Identification of biological markers that may predict response to chemotherapy would allow the individualization of treatment by enabling selection of patients most likely to benefit from chemotherapy. The aims of this study were to determine whether p53 mutation status and p53 and p33ING1b protein expression can predict which patients with Dukes'C colorectal cancer following curative surgical resection respond to adjuvant chemotherapy with 5-fluorouracil (5-FU). Method: Patients with Dukes'C colorectal cancer (n = 41) were studied. DNA was extracted and analysed for p53 mutation using PCR-based direct DNA sequencing. Tumours were analysed for p53 protein expression by immunohistochemistry using DO-7 monoclonal antibody and for p33ING1b protein expression using GN1 monoclonal antibody. Results: There was a significant association between p53 mutation status analysed by gene sequencing and overall and metastasis-free survival (P = 0.03 and 0.004, respectively, log-rank test). By contrast, no significant correlation was found between p53 and p33ING1b protein expression and overall or metastasis-free survival. Conclusion: In patients with Dukes' C colorectal cancer who underwent curative surgical resection of the primary tumour, followed by 5-FU-based adjuvant chemotherapy, p53 mutation status as assessed by gene sequencing is a significant predictor of overall and metastasis-free survival. © 2008 The Association of Coloproctology of Great Britain and Ireland.


Publication metadata

Author(s): Ahmed I, Kelly S, Anderson JJ, Angus B, Challen C, Lunec J

Publication type: Article

Publication status: Published

Journal: Colorectal Disease

Year: 2008

Volume: 10

Issue: 4

Pages: 344-351

ISSN (print): 1462-8910

ISSN (electronic): 1463-1318

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1111/j.1463-1318.2007.01317.x

DOI: 10.1111/j.1463-1318.2007.01317.x

PubMed id: 17949449


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