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Biosystematics of alkaliphilic streptomycetes isolated from seven locations across a beach and dune sand system

Lookup NU author(s): Dr Sanjay Babu, Professor Michael Goodfellow

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Abstract

Alkaliphilic streptomycetes were isolated from composite sand samples collected from six out of seven locations across a beach and dune sand system using starch-casein-nitrate agar supplemented with cycloheximide and buffered to pH 10.5. The isolates had colonial and chemotaxonomic properties consistent with their classification in the genus Streptomyces. They were assigned to 49 multimembered and 114 single-membered colour-groups given their ability to produce pigments on oatmeal and peptone-yeast-extract-iron agars and to corresponding taxa based on whole-genome rep-PCR banding patterns. Twenty-four isolates representing the colour and rep-PCR groups grew well from pH 5 to 11, and optimally at pH 9, as did phylogenetically close members of the Streptomyces griseus 16S rRNA gene clade. One hundred and twelve representative alkaliphilic streptomycetes formed a heterogeneous but distinct clade in the Streptomyces 16S rRNA gene tree. A 3-dimensional representation of 16S rRNA sequence data showed that the alkaliphilic streptomycetes formed a distinct group in multidimensional taxospace. It is evident that alkaliphilic streptomycetes are common in the beach and dune sand system and that representatives of this community form new centers of taxonomic variation within the genus Streptomyces that can be equated with species. © Springer Science+Business Media B.V. 2008.

Publication metadata

Author(s): Antony-Babu S, Goodfellow M

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Year: 2008

Volume: 94

Issue: 4

Pages: 581-591

Print publication date: 01/01/2008

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer Netherlands

URL: http://dx.doi.org/10.1007/s10482-008-9277-4

DOI: 10.1007/s10482-008-9277-4

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