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Binding of liver derived, low density hepatitis C virus to human hepatoma cells

Lookup NU author(s): Dr Soren Nielsen, Siti Ibrahim, Professor Margaret Bassendine, Professor Geoff Toms

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Abstract

HCV recovered from low density fractions of infected blood is associated with lipid and host apo-lipoproteins in lipo-viro-particles (LVP). It has been proposed that these particles are capable of binding and entering hepatocytes by viral glycoprotein independent mechanisms utilizing uptake pathways of normal host lipoproteins after binding to cell surface glycosaminoglycans (GAG), the low density lipoprotein receptor (LDL-r) or scavenger receptor B1 (SR-B1). In this study binding to human hepatoma cells of HCV low density RNA containing particles, semi-purified from macerates of infected human liver, is compared with that of normal host low density lipoprotein (LDL). Binding of both LDL and HCV low density RNA containing particles paralleled LDL-r but not SR-B1 expression on the recipient cells. Binding of both particle types was sensitive to suramin at O°C but less so at 37°C suggesting that they both bind initially to GAG but, at 37°C, are internalized or transferred to a suramin resistant receptor. Suramin resistant uptake of both particles was blocked in the presence of excess LDL or oxidized LDL. However, whilst LDL uptake was blocked by anti-apoB-100, HCV low density RNA uptake was enhanced by anti-apoB 100 and further enhanced by a cocktail of anti-apo-B100 and anti-apoE. Preincubation of HCV low density RNA containing particles with antibodies to the E2 glycoprotein had little or no effect on uptake. These data indicate that whilst liver derived HCV RNA containing particles are taken up by HepG2 cells by a virus glycoprotein independent mechanism, the mechanism differs from that of LDL uptake. © 2008 Wiley-Liss, Inc.


Publication metadata

Author(s): Martin C, Nielsen SU, Ibrahim SF, Bassendine MF, Toms GL

Publication type: Article

Publication status: Published

Journal: Journal of Medical Virology

Year: 2008

Volume: 80

Issue: 5

Pages: 816-823

ISSN (print): 0146-6615

ISSN (electronic): 1096-9071

Publisher: Wiley

URL: http://dx.doi.org/10.1002/jmv.21150

DOI: 10.1002/jmv.21150

PubMed id: 18360895


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