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CD19 is essential for B cell activation by promoting B cell receptor-antigen microcluster formation in response to membrane-bound ligand

Lookup NU author(s): Professor Kevin Marchbank

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Abstract

Here we describe the spatiotemporal architecture, at high molecular resolution, of receptors and signaling molecules during the early events of mouse B cell activation. In response to membrane-bound ligand stimulation, antigen aggregation occurs in B cell antigen receptor (BCR) microclusters containing immunoglobulin (Ig) M and IgD that recruit the kinase Syk and transiently associate with the coreceptor CD19. Unexpectedly, CD19-deficient B cells were significantly defective in initiation of BCR-dependent signaling, accumulation of downstream effectors and cell spreading, defects that culminated in reduced microcluster formation. Hence, we have defined the dynamics of assembly of the main constituents of the BCR 'signalosome' and revealed an essential role for CD19, independent of the costimulatory molecule CD21, in amplifying early B cell activation events in response to membrane-bound ligand stimulation.


Publication metadata

Author(s): Depoil D, Fleire S, Treanor BL, Weber M, Harwood NE, Marchbank KL, Tybulewicz VLJ, Batista FD

Publication type: Article

Publication status: Published

Journal: Nature Immunology

Year: 2008

Volume: 9

Issue: 1

Pages: 63-72

ISSN (print): 1529-2908

ISSN (electronic): 1529-2916

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/ni1547

DOI: 10.1038/ni1547

PubMed id: 18059271


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