Browse by author
Lookup NU author(s): Professor Gerard Stansby
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
The mechanism of thrombogenicity in hyperhomocysteinemia remains controversial. The authors investigated the association between elevated plasma homocysteine levels, platelet function, and blood coagulation. Blood was collected from healthy subjects and patients with critical limb ischemia. Basal platelet counts and platelet aggregation as well as flow cytometry were performed to assess spontaneous- and agonist-induced platelet aggregation as well as P-selectin and Glycoprotein IIb/IIIa expression at different homocysteine concentrations. Thromboelastography was performed, and platelet shape change was assessed, using a channelyzer, by measuring median platelet volume. Lactate dehydrogenase was measured, to indirectly assess red blood cell membrane integrity, after homocysteine exposure. The study results suggest that platelet activation and hypercoagulability occur after exposure to homocysteine, especially in patients with critical limb ischemia. Homocysteine concentrations of approximately 50 μmol/L appear to be the level at which these changes occur in vitro, and this effect on platelets appears to be indirect. © 2008 Sage Publications.
Author(s): Mohan IV, Jagroop IA, Mikhailidis DP, Stansby GP
Publication type: Article
Publication status: Published
Journal: Clinical and Applied Thrombosis/Hemostasis
ISSN (print): 1076-0296
ISSN (electronic): 1938-2723
Publisher: Sage Publications, Inc.
PubMed id: 18160593
Altmetrics provided by Altmetric