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The genotypic characterisation of Acanthamoeba isolates from human ocular samples

Lookup NU author(s): Dr Christophe Noel


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Background: We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis. Methods: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis. Results: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis. Conclusion: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.

Publication metadata

Author(s): Yera H, Zamfir O, Bourcier T, Viscogliosi E, Noel C, Dupouy-Camet J, Chaumeil C

Publication type: Article

Publication status: Published

Journal: British Journal of Ophthalmology

Year: 2008

Volume: 92

Issue: 8

Pages: 1139-1141

ISSN (print): 0007-1161

ISSN (electronic): 1468-2079

Publisher: BMJ Group


DOI: 10.1136/bjo.2007.132266

PubMed id: 18586902


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