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Dissection of a co-translational nascent chain separation event

Lookup NU author(s): Dr Victoriya Doronina, Pamila Sharma, Dr Jeremy Brown

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Abstract

Some RNA and protein sequences are capable of directing changes to the course of translation from that expected from the mRNA sequence, and this process is termed translational 'recoding'. 'CHYSEL' peptides are ε119-amino-acid sequences found in many viral genomes. When translated at internal portions of polypeptides, they yield co-translational separation of the nascent chain at their C-termini. We dissected the reaction promoted by CHYSEL sequences using yeast genetics and in vitro translation systems. Our results indicate that the reaction occurs within the peptidyltransferase centre of the ribosome where the nascent chain is hydrolytically released from tRNA despite the presence of further sense codons. © The Authors Journal compilation © 2008 Biochemical Society.

Publication metadata

Author(s): Doronina VA, de Felipe P, Wu C, Sharma P, Sachs MS, Ryan MD, Brown JD

Publication type: Article

Publication status: Published

Journal: Biochemical Society Transactions

Year: 2008

Volume: 36

Issue: 4

Pages: 712-716

Print publication date: 01/08/2008

ISSN (print): 0300-5127

ISSN (electronic): 1470-8752

Publisher: Portland Press Ltd.

URL: http://dx.doi.org/10.1042/BST0360712

DOI: 10.1042/BST0360712

PubMed id: 18631146

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