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Dynamic Control of the DNA Replication Initiation Protein DnaA by Soj/ParA

Lookup NU author(s): Professor Heath Murray, Professor Jeff ErringtonORCiD


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Regulation of DNA replication and segregation is essential for all cells. Orthologs of the plasmid partitioning genes parA, parB, and parS are present in bacterial genomes throughout the prokaryotic evolutionary tree and are required for accurate chromosome segregation. However, the mechanism(s) by which parABS genes ensure proper DNA segregation have remained unclear. Here we report that the ParA ortholog in B. subtilis (Soj) controls the activity of the DNA replication initiator protein DnaA. Subcellular localization of several Soj mutants indicates that Soj acts as a spatially regulated molecular switch, capable of either inhibiting or activating DnaA. We show that the classical effect of Soj inhibiting sporulation is an indirect consequence of its action on DnaA through activation of the Sda DNA replication checkpoint. These results suggest that the pleiotropy manifested by chromosomal parABS mutations could be the indirect effects of a primary activity regulating DNA replication initiation. © 2008 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Murray HD, Errington J

Publication type: Article

Publication status: Published

Journal: Cell

Year: 2008

Volume: 135

Issue: 1

Pages: 74-84

ISSN (print): 0092-8674

ISSN (electronic): 1097-4172

Publisher: Cell Press


DOI: 10.1016/j.cell.2008.07.044


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Funder referenceFunder name
European Molecular Biology Organization (EMBO)
Human Frontier Science Program (HFSP)