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The construction and in vitro testing of photo-activatable cancer targeting folated anti-CD3 conjugates

Lookup NU author(s): Dr Stephen Thompson, John Dessi, Emeritus Professor Colin Self

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Abstract

The construction and in vitro testing of a photo-activatable anti-tumour immuno-regulatory antibody is described. In this 'cloaked' folated anti-CD3 antibody conjugate, the folate portion of the conjugate is free to bind to folate receptor expressing cancer cells, whilst the anti-CD3 activity is effectively rendered inert by a coating of photo-labile 2-nitrobenzyl groups. On irradiation with UV-A light the activity of the anti-CD3 antibody is restored, not only when it is required, but more importantly, only where it is required. The conjugate can then attract killer T-cells to the surface of the tumour cells and kill them. Unirradiated normal tissues, to which the conjugate has been targeted by specific and non-specific binding, remain unharmed. We believe that these 'photo-switchable' conjugates could be used to markedly improve the targeting of the immune response to folate receptor (FR) expressing ovarian and breast cancers whilst minimising the side effects in the rest of the body. © 2007 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Thompson S, Dessi J, Self CH

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 2008

Volume: 366

Issue: 2

Pages: 526-531

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.bbrc.2007.11.158

DOI: 10.1016/j.bbrc.2007.11.158

PubMed id: 18068664

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