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Molecular microbial ecology of nitrification in an activated sludge process treating refinery wastewater

Lookup NU author(s): Stuart Ballinger, Professor Ian Head, Professor Thomas CurtisORCiD


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The diversity of autotrophic ammonia-oxidizing bacteria (AOB) of the b-subdivision of the-class Proteobacteria was investigated in a laboratory-scale denitrification-nitrification bioreactor (DNB) treating a synthetic waste stream. 16S ribosomal RNA (rRNA) gene sequences were amplified from DNA extracted from the oxic DNB sludge. Comparative analysis of the rRNA sequences revealed considerable diversity among the AOB-like sequences. The majority of sequences recovered were related to Nitrosomonas spp. but a smaller number of Nitrosospira-like sequences were obtained. Since different AOB may have different kinetic properties the high diversity of AOB, even in a simple laboratory biotreater treating a simple waste stream, has important implications for the operation of nitrifying wastewater treatment processes. (C) 1998 IAWQ. Published by Elsevier Science Ltd.

Publication metadata

Author(s): Ballinger SJ, Head IM, Curtis TP, Godley AR

Publication type: Article

Publication status: Published

Journal: Water Science and Technology

Year: 1998

Volume: 37

Issue: 4-5

Pages: 105-108

Print publication date: 24/07/1998

ISSN (print): 0273-1223

ISSN (electronic):

Publisher: IWA Publishing


DOI: 10.1016/S0273-1223(98)00091-2


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