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In situ detection of rhodococci associated with activated sludge foams

Lookup NU author(s): Professor Russell DavenportORCiD, Professor Thomas CurtisORCiD, Jeannie Upton

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Abstract

Genus-specific 16S rRNA targeted oligonucleotide probes, Rco1 and Rco2, were designed and used to detect rhodococci in activated sludge foam samples by confocal laser scanning microscopy. Pure cultures were used to find the optimal hybridisation conditions which were determined by comparing the mean fluorescent intensities of target and non-target cells from images captured using a confocal laser scanning microscope (CLSM). The combination of fluorescent in situ hybridisation with rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy provides an effective way of detecting rhodococci in environmental samples.

Publication metadata

Author(s): Davenport RJ, Elliott JN, Curtis TP, Upton J

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 1998

Volume: 74

Issue: 1-3

Pages: 41-48

Print publication date: 01/07/1998

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer Netherlands

URL: http://dx.doi.org/10.1023/A:1001795609982

DOI: 10.1023/A:1001795609982

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