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Differential regulation of syndecan expression by osteosarcoma cell lines in response to cytokines but not osteotropic hormones

Lookup NU author(s): Dr Mark Birch


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Bone cells are regulated by interactions with both growth factors and components of the extracellular matrix (ECM), Syndecans are cell-surface heparan sulfate proteoglycans known to play a role in cell adhesion and migration, and binding of growth factors. This study was performed to investigate the expression of syndecans by osteoblasts. Reverse transcription-linked polymerase chain reaction (RT-PCR) and Northern analysis detected syndecan transcripts in the human osteosarcoma cell lines MG-63, TE-85, SaOS-2, and U2OS; human osteoblast-like cells; rat calvarial osteoblasts; and in human bone. Western blot analysis of proteoglycans from MG-63 and TE-85 cells detected multiple heparan sulfate proteoglycan core proteins consistent with syndecan expression, Regulation of syndecan-1, -2, and -4 expression was investigated in TE-85, MG-63, and SaOS-2 cells, in response to interleukin (IL)-1 beta, and IL-6, parathyroid hormone [PTH(1-34)], and 1,25(OH)(2)-vitamin D-3. Northern analysis demonstrated that in the osteosarcoma cell lines there was no regulation of syndecan transcript levels in response to PTH(1-34) or 1,25(OH)(2)-vitamin D-3 for 24 or 48 h. In contrast, when MG-63 and SaOS-2 cells were incubated with IL-1 beta (0.01-10 ng/mL) and IL-6 (0.1-50 ng/mL) there was a dose-dependent decrease in mRNA levels for syndecan-1 and 2 at 24 and 48 h, but in response to IL-1 beta upregulation in the levels of syndecan-4 transcripts, In addition, Northern analysis was performed on RNA isolated from neonatal rat calvarial osteoblasts cultured under conditions that promote osteogenesis for 0, 5, 13, 21, and 35 days. Syndecan-1 expression was observed to decrease during the culture period, syndecan-2 transcript levels increased, and there appeared to be no overall change in syndecan-4 levels. Controlled expression of syndecans by cells of the osteoblast lineage may be important in the regulation of osteoblastic proliferation and differentiation.

Publication metadata

Author(s): Birch MA, Skerry TM

Publication type: Article

Publication status: Published

Journal: Bone

Year: 1999

Volume: 24

Issue: 6

Pages: 571-578

Print publication date: 01/06/1999

ISSN (print): 8756-3282

ISSN (electronic):

Publisher: Elsevier


DOI: 10.1016/S8756-3282(99)00088-5


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