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Lookup NU author(s): Dr Zahed Chowdhury,
Dr Gavin Spickett,
Professor Robert Wilkinson
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Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). There is controversy over the value of formalin fixation of neutrophils in differentiating antibodies giving selective or preferential reaction with the nuclear or perinuclear area of neutrophils. In a comparative study of 77 sera, formalin fixation caused inconsistency, nonspecific effects, and false positivity owing to enhanced fluorescence, If formalin fixed neutrophils are used in the routine diagnostic laboratory, this will add confusion to the interpretation of the ANCA assay.
Author(s): Chowdhury SMZ, Broomhead V, Spickett GP, Wilkinson R
Publication type: Article
Publication status: Published
Journal: Journal of Clinical Pathology
Print publication date: 01/06/1999
ISSN (print): 0021-9746
Publisher: B M J Group
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