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Lookup NU author(s): Dr Simon Rees,
Emeritus Professor Peter Olive
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A new simplified technique for the extraction of Nereis vitellin (NV) from oocytes using affinity chromatography has been developed. This protein extract has been utilised in a new in vitro fluorescence assay to investigate the binding and incorporation of yolk protein into the oocytes of Nereis (Neanthes) virens. Protein incorporation was investigated in relation to the environmental conditions experienced by the animals. Three different environmental regimes were investigated: LD 16:8 and 10 degrees C, LD 8:16 and 10 degrees C, decerebrated in LD 8:16 and 10 degrees C. Fluorescence per oocyte (f/o) was measured at the start of the experiment and after 14, 21 and 42 days for each experimental animal. After 42 days, the oocytes from the animals maintained in LD 16:8 showed little increase in f/o whilst the oocytes from the animals in LD 8:16 did. The oocytes from the decerebrated animals showed an initial large increase in f/o up to 21 days but this had decreased by 42 days. These results demonstrate an influence of external photoperiod on the cellular mechanism of protein incorporation by the oocyte and could be used to assist in the identification of the hormones that are involved in the regulation of oocyte growth in the nereid polychaetes. (C) 1999 Elsevier Science Inc. All rights reserved.
Author(s): Rees SW; Olive PJW
Publication type: Article
Publication status: Published
Journal: Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology
Print publication date: 11/06/1999
ISSN (print): 1095-6433
Publisher: Elsevier Inc.
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