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Lookup NU author(s): Dr John Perry,
Dr Katherine Orr
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We describe the synthesis of a new substrate for the detection of bacterial beta-galactosidase. This substrate, alizarin-beta-d-galactoside, is readily hydrolysed to release alizarin which complexes with various metal ions to form brightly coloured chelates. A total of 367 strains of Gram-negative bacteria were examined for their ability to hydrolyse three chromogenic substrates: alizarin-beta-d-galactoside (Aliz-gal), cyclohexenoesculetin-beta-d-galactoside (CHE-gal) and 5-bromo-4-chloro-3-indolyl-beta-d-galactoside (X-gal). A total of 182 strains (49.6%) were found to hydrolyse at least one of the three substrates. All of these 182 strains (100%) hydrolysed Aliz-gal whereas only 170 (93.4%) and 173 (95.1%) hydrolysed CHE-gal and X-gal, respectively. We conclude that alizarin-beta-d-galactoside is a highly sensitive substrate for the demonstration of beta-galactosidase.
Author(s): James AL, Perry JD, Chilvers K, Robson IS, Armstrong L, Orr KE
Publication type: Article
Publication status: Published
Journal: Letters in Applied Microbiology
ISSN (print): 0266-8254
ISSN (electronic): 1472-765X
Publisher: Wiley-Blackwell Publishing Ltd.
PubMed id: 10792658
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