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Lookup NU author(s): Dr Olivier Sparagano
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Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V alginolyticus, V anguillarum and for a cluster of taxonomically related species: V carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.
Author(s): Sparagano OAE, Robertson PAW, Purdom I, McInnes J, Li Y, Yu DH, Du ZJ, Xu HS, Austin B
Editor(s): Gibbs, E.P.J., Bokma, B.H.
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: The Domestic Animal/Wildlife Interface: Issues for Disease Control, Conservation, Sustainable Food Production, and Emerging Diseases
Year of Conference: 2002
Pages: 60-65
ISSN: 0077-8923
Publisher: New York Academy of Sciences
Library holdings: Search Newcastle University Library for this item
Series Title: Annals of the New York Academy of Sciences
ISBN: 9781573314381
