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PCR and molecular detection for differentiating Vibrio species

Lookup NU author(s): Dr Olivier Sparagano


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Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V alginolyticus, V anguillarum and for a cluster of taxonomically related species: V carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

Publication metadata

Author(s): Sparagano OAE, Robertson PAW, Purdom I, McInnes J, Li Y, Yu DH, Du ZJ, Xu HS, Austin B

Editor(s): Gibbs, E.P.J., Bokma, B.H.

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: The Domestic Animal/Wildlife Interface: Issues for Disease Control, Conservation, Sustainable Food Production, and Emerging Diseases

Year of Conference: 2002

Pages: 60-65

ISSN: 0077-8923

Publisher: New York Academy of Sciences

Library holdings: Search Newcastle University Library for this item

Series Title: Annals of the New York Academy of Sciences

ISBN: 9781573314381