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Lookup NU author(s): Robert Griffiths,
Professor Anthony O'Donnell
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A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.
Author(s): Griffiths RI; O'Donnell AG; Whiteley AS; Bailey MJ
Publication type: Article
Publication status: Published
Journal: Applied and Environmental Microbiology
ISSN (print): 0099-2240
ISSN (electronic): 1098-5336
Publisher: American Society for Microbiology
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