Toggle Main Menu Toggle Search

Open Access padlockePrints

Impaired RNA splicing of 5 '-regulatory sequences of the astroglial glutamate transporter EAAT2 in human astrocytoma

Lookup NU author(s): Dr Thomas Meyer


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


A loss of the glutamate transporter EAAT2 has been reported in the neoplastic transformation of astrocytic cells and astrocytoma. The RNA expression of EAAT2 and five 5'-regulatory splice variants was investigated to identify alterations of the post-transcriptional EAAT2 gene regulation in human astrocytic tumours. Three known (EAAT2, HBGTII, and HBGTIIC) and two novel (EAAT2/3 and EAAT2/31) EAAT2 transcripts originating from alternative splicing of 5'-regulatory sequences were subject to an RNA expression analysis using reverse transcription and competitive PCR. Specimens of astrocytoma World Health Organisation (WHO) grade I-IV in 14 patients and control brain tissue obtained from three normal persons were studied. The main EAAT2 RNA was found to be equally expressed in normal human brain and astrocytic tumour samples. By contrast, the expression pattern of four 5'-variants of the transporter transcript was altered in the investigated series of astrocytoma compared with normal brain. HBGTII, HBGTIIC, and EAAT2/3 were amplified from seven and four tumours and one sample, respectively. EAAT2/31 was expressed in none of the tumour specimens studied. In conclusion, in astrocytic. tumours of different histopathological grades there was a substantial reduction of RNA splicing events in EAAT2. The impairment of EAAT2 splicing indicates an altered expression which is not primarily involved in the tumorigenesis but may contribute to some biological properties of astrocytoma such as oedema, necrosis, and tumour related seizures.

Publication metadata

Author(s): Munch C, Penndorf A, Schwalenstocker B, Troost D, Ludolph AC, Ince P, Meyer T

Publication type: Article

Publication status: Published

Journal: Journal of Neurology, Neurosurgery and Psychiatry

Year: 2001

Volume: 71

Issue: 5

Pages: 675-678

ISSN (print): 0022-3050

ISSN (electronic):

Publisher: BMJ Group


DOI: 10.1136/jnnp.71.5.675


Altmetrics provided by Altmetric