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Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases

Lookup NU author(s): Dr Christopher Graham, Jane Heatherington


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A novel real-time PCR-hybridization assay was developed for the rapid (<1 h) detection of penicillin susceptibility in Streptococcus pneumoniae. When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitive S. pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases.

Publication metadata

Author(s): Kearns AM, Graham C, Burdess D, Heatherington J, Freeman R

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Microbiology

Year: 2002

Volume: 40

Issue: 2

Pages: 682-684

ISSN (print): 0095-1137

ISSN (electronic): 1098-660X

Publisher: American Society for Microbiology


DOI: 10.1128/JCM.40.2.682-684.2002


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