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The reliability of marker/reporter genes in biocontrol studies with B-subtilis

Lookup NU author(s): Professor Carlo Leifert

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Abstract

A commercially available biocontrol agent, Bacillus subtilis MBI600, was transformed with plasmids containing a luciferase luxAB fusion cassette under the control of a constitutive promoter, as well as two promoter-less plasmids, containing either a luxAB fusion cassette or luxA and luxB genes. Introduction of these genes did not alter the growth rate, but subsequent expression of the luciferase enzyme resulted in a consistently compromised in vitro biocontrol activity. The expressed luciferase gene product (rather then the insertion of the novel genes) appeared to have undesired side effects on the phenotype of the transformed organism, demonstrating why caution should be exercised when using marker/reporter gene systems for investigating biocontrol.

Publication metadata

Author(s): Leifert C; Knox OGG; Killham K

Publication type: Article

Publication status: Published

Journal: Biocontrol Science and Technology

Year: 2002

Volume: 12

Issue: 5

Pages: 637-641

Print publication date: 28/06/2010

ISSN (print): 0958-3157

ISSN (electronic): 1360-0478

Publisher: Taylor & Francis Ltd.

URL: http://dx.doi.org/10.1080/0958315021000016306

DOI: 10.1080/0958315021000016306

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