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Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of phenols

Lookup NU author(s): Dr Seung Chang, Emeritus Professor Calum McNeilORCiD


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A new disposable amperometric bi-enzyme sensor system for detecting phenols has been developed. The phenol sensor developed uses horseradish peroxidase modified screen-printed carbon electrodes (HRP-SPCEs) coupled with immobilized tyrosinase prepared using poly(carbamoylsulfonate) (PCs) hydrogels or a poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) matrix. Optimization of the experimental parameters has been performed with regard to buffer composition, pH, operating potential and storage stability. A co-operative reaction involving tyrosinase and HRP occurs at a potential of -50 mV versus Ag/AgCl without the requirement for addition of extraneous H2O2, thus, resulting in a very simple and efficient system. Comparison of the electrode responses with the 4-aminoantipyrine standard method for phenol sample analysis indicated the feasibility of the disposable sensor system for sensitive 'in-field' determination of phenols. The most sensitive system was the tyrosinase immobilized HRP-SPCE using PCs, which displayed detection limits for phenolic compounds in the lower nanomolar range e.g. 2.5 nM phenol, 10 nM catechol and 5 nM p-cresol. (C) 2002 Elsevier Science B.V. All rights reserved.

Publication metadata

Author(s): Chang SC, Rawson K, McNeil CJ

Publication type: Article

Publication status: Published

Journal: Biosensors and Bioelectronics

Year: 2002

Volume: 17

Issue: 11-12

Pages: 1015-1023

ISSN (print): 0956-5663

ISSN (electronic): 1873-4235

Publisher: Elsevier BV


DOI: 10.1016/S0956-5663(02)00094-5


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