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Mu class glutathione S-transferase mRNA isoform expression in acute lymphoblastic leukaemia

Lookup NU author(s): Dr Pamela Kearns, Professor Zofia Chrzanowska-Lightowlers, Emeritus Professor Andy Hall

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Abstract

Glutathione S-transferases (GSTs) are implicated in cytotoxic drug resistance in leukaemia. In a previous study, expression of mu class GST (GSTM) was associated with poor prognosis in childhood acute lymphoblastic leukaemia (ALL), however, that study did not differentiate between individual GSTM isoforms. This study, therefore, investigated individual GSTM isoform expression in ALL blasts at the mRNA level. Leukaemic blasts from 21 children with ALL were studied. Interindividual variation in the pattern of GSTM mRNA isoform expression was demonstrated. GSTM2 transcript was expressed in all patients in contradistinction to GSTM5, which was not detected in any sample. GSTM3 and GSTM4 expression varied between individuals, with GSTM3 expressed in 62% and GSTM4 in 24% of patients. Lymphoblast expression of GSTM3 was positively related to good prognosis whereas expression of GSTM4 was not related to clinical outcome in this small cohort. No relationship was demonstrated with established indicators of prognosis, including sex, age, immunophenotype and presenting white cell count. The results suggest that expression of GSTM3 may play a role in determining prognosis in childhood ALL and could provide more information for accurate stratification of treatment. Further studies are required to determine whether there is a causal relationship between GSTM3 expression and clinical outcome.


Publication metadata

Author(s): Kearns PR, Chrzanowska-Lightowlers ZMA, Pieters R, Veerman A, Hall AG

Publication type: Article

Publication status: Published

Journal: British Journal of Haematology

Year: 2003

Volume: 120

Issue: 1

Pages: 80-88

ISSN (print): 0007-1048

ISSN (electronic): 1365-2141

URL: http://dx.doi.org/10.1046/j.1365-2141.2003.04039.x

DOI: 10.1046/j.1365-2141.2003.04039.x

PubMed id: 12492580


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