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Direct dialysis quantification: Investigation of the impact of dialysate preservation techniques on solute assays

Lookup NU author(s): Dr Suren Kanagasundaram


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Aims: Urease-producing microorganisms may lower urea nitrogen (UN) during dialysate-side dosing. We investigated the impact of 3 proven preservatives (acetic acid, ceftazidime, thimerosal) on UN concentration, and the concentrations of creatinine (CR) and beta(2)-microglobulin (beta(2)M). Methods: The UN, CR and beta(2)M concentrations were assayed in 3 separate aliquots from 20 spent dialysate samples (ceftazidime, 125 mg/l, or 1% thimerosal, 1 ml/l, vs. control). The beta(2)M concentration was assayed in 10 further spent dialysate collections (concentrated glacial acetic acid, 5 ml/l, vs. control). Solute concentrations were compared with the concordance correlation coefficient (r(c)). Results: Ceftazidime and thimerosal had little effect on the concentrations of UN and CR (r(c) >0.97). For the beta(2)M concentration, agreement remained good (r(c) >0.96) for ceftazidime and thimerosal (although the former tended to lower concentrations) but acetic acid was less optimal (r(c) = 0.893). Conclusions: Ceftazidime and thimerosal may be used as dialysate preservatives without affecting the UN or CR concentrations. Thimerosal is to be preferred when studying beta(2)M. Acetic acid produces unacceptable inaccuracy when measuring beta(2)M. Copyright (C) 2004 S. Karger AG, Basel.

Publication metadata

Author(s): Kanagasundaram NS, Posch A, Larive AB, Paganini EP, Van Lente F

Publication type: Article

Publication status: Published

Journal: Blood Purification

Year: 2004

Volume: 22

Issue: 5

Pages: 435-439

Print publication date: 01/01/2004

ISSN (print): 0253-5068

ISSN (electronic): 1421-9735

Publisher: S. Karger AG


DOI: 10.1159/000080727


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Funder referenceFunder name
R01 DK 53411-01A1NIDDK NIH HHS