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Characterization of a truncated lipoarabinomannan from the actinomycete Turicella otitidis

Lookup NU author(s): Dr Jerome Nigou

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Abstract

Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the structural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) has been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an (alpha 1-->6)-linked mannan core and substituted with terminal-arabinosyl branches. Thus, TotLAM has a novel truncated LAM structure. Using the human monocytic THP-1 cell line, it was found that TotLAM exhibited only minimal ability to induce tumor necrosis factor alpha. These findings contribute further to our understanding of actinomycete LAM diversity and allow further speculation as to the correlation between LAM structure and the immunomodulatory activities of these lipoglycans.

Publication metadata

Author(s): Gilleron M, Garton NJ, Nigou J, Brando T, Puzo G, Sutcliffe IC

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 2005

Volume: 187

Issue: 3

Pages: 854-861

ISSN (print): 0021-9193

ISSN (electronic): 1098-5530

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/JB.187.3.854-861.2005

DOI: 10.1128/JB.187.3.854-861.2005

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