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Lookup NU author(s): Emeritus Professor Drew Rowan,
Professor David Deehan,
Emeritus Professor Tim Cawston
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Background: The synergistic degradation of cartilage by oncostatin M (OSM) in combination with either interleukin 1 (IL1) or tumour necrosis factor alpha (TNF alpha) has been previously demonstrated using bovine nasal cartilage (BNC). Objectives: (a) To investigate if human nasal cartilage (HNC) responds in the same way as BNC to these cytokine combinations, particularly in collagen degradation. (b) To compare the response of human nasal and articular cartilages. Methods: Collagen release was assessed by measuring the hydroxyproline content of culture supernatants and proteoglycan release by the dimethylmethylene blue assay. Matrix metalloproteinase (MMP)-1, MMP13, and tissue inhibitor of metalloproteinase 1 release were measured by specific enzyme linked immunosorbent assays (ELISAs), and collagenolytic activity was measured by a bioassay using radiolabelled collagen. Results: OSM in combination with either IL1 or TNF alpha acted synergistically to induce collagenolysis from HNC, with a maximum of 79% collagen release. This degradation strongly correlated with MMP-1 and MMP-13 levels and collagenolytic activity. Conclusion: Collagen release from human cartilage is marked and implicates both MMP-1 and MMP-13 in the synergistic degradation of human cartilage by OSM in combination with either IL1 or TNF alpha. HNC responds in the same way as BNC, thus validating the bovine cartilage degradation assay as a model relevant to human disease.
Author(s): Morgan TG, Rowan AD, Dickinson SC, Jones D, Hollander AP, Deehan D, Cawston TE
Publication type: Article
Publication status: Published
Journal: Annals of the Rheumatic Diseases
ISSN (print): 0003-4967
ISSN (electronic): 1468-2060
Publisher: BMJ Group
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