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Lookup NU author(s): Emeritus Professor Arthur Covington
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A reference method is described for the determination of the substance concentration of ionized calcium in plasma by which ionized calcium (free or unbound) may be reliably determined on the basis of calibration with aqueous solutions with known concentration of ionized calcium. The composition of the calibration solutions is chosen such that the activity coefficient of the calcium ion is assumed to be identical both in the calibration solutions and in "normal" plasma, i.e. by convention, the ionic strength (I-m) is 0.160 mol/kg. The convention is adopted of reporting ionized calcium measurements as concentration expressed as mmol/l. The proposed reference method for ionized calcium measurement in plasma is based on the use of a cell consisting of an external reference electrode with a saturated potassium chloride liquid/liquid junction in combination with a calcium ion-selective membrane electrode of defined construction and performance. Procedures for using the reference cell and a protocol for sample measurement are described. The preparation of the calibration solutions to be used are described in detail in Appendix A, secondary calibration solutions and check standards in Appendix B, and reference cell vessel design in Appendix C.
Author(s): Burnett RW, Christiansen TF, Covington AK, Fogh-Andersen N, Kulpmann WR, Lewenstam A, Maas AHJ, Muller-Plathe O, Sachs C, Siggaard-Andersen O, VanKessel AL, Zijlstra WG
Publication type: Article
Publication status: Published
Journal: Clinical Chemistry and Laboratory Medicine
Year: 2000
Volume: 38
Issue: 12
Pages: 1301-1314
ISSN (print): 1434-6621
ISSN (electronic): 1437-4331
Publisher: Walter de Gruyter GmbH & Co.
URL: http://dx.doi.org/10.1515/CCLM.2000.206
DOI: 10.1515/CCLM.2000.206
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