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Lookup NU author(s): Dr John Perry,
Professor Lyle Armstrong,
Professor Kate Gould
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We describe the synthesis of a new substrate for the detection of beta -galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta -D-galactopyranoside (X-Gal) and cyclohexenoesculetin-beta -D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta -D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta -galactosidase.
Author(s): James AL, Chilvers KF, Perry JD, Armstrong L, Gould FK
Publication type: Article
Publication status: Published
Journal: Applied and Environmental Microbiology
ISSN (print): 0099-2240
ISSN (electronic): 1098-5336
Publisher: American Society of Microbiology
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