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Fluorogenic substrates for the detection of microbial nitroreductases

Lookup NU author(s): Dr John Perry, Professor Kate Gould

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Abstract

Aims: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. Methods and Results: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. Conclusions, Significance and Impact of the Study: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.

Publication metadata

Author(s): James AL, Perry JD, Jay C, Monget D, Rasburn JW, Gould FK

Publication type: Article

Publication status: Published

Journal: Letters in Applied Microbiology

Year: 2001

Volume: 33

Issue: 6

Pages: 403-408

ISSN (print): 0266-8254

ISSN (electronic): 1472-765X

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1472-765X.2001.01021.x

DOI: 10.1046/j.1472-765X.2001.01021.x

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