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Upregulation of neuronal nicotinic receptor subunits alpha 4, beta 2, and alpha 7 in transgenic mice overexpressing human acetylcholinesterase

Lookup NU author(s): Mary Johnson, Dr Jennifer Court, Emeritus Professor Elaine Perry

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Abstract

Neuronal nicotinic receptor binding sites as well as mRNA levels encoding for subunits alpha4, beta2, and alpha7 were analysed in 3-mo-old transgenic mice generated with a neuronal overexpression of human acetylcholinesterase and in age-matched controls. The acetylcholinesterase transgenic mice display progressive cognitive impairment in spatial learning and memory. We here report a significantly increased [H-3]epibatidine and [I-125] alphabungarotoxin binding in the cortex and the caudate putamen of these mice. Quantitative in situ hybridization showed significant upregulation of mRNA corresponding to the nicotinic receptor subunits alpha4, beta2, and alpha7 in various brain regions in the transgenic mice compared to nontransgenic controls. Our results suggest that disruption of balanced cholinergic transmission by constitutive overexpression of acetylcholinesterase is accompanied by variable upregulation of several nicotinic receptor subtypes, in particular these associated with cholinergic terminals participating in compensatory response.

Publication metadata

Author(s): Svedberg MM, Svensson AL, Johnson M, Lee M, Cohen O, Court J, Soreq H, Perry E, Nordberg A

Publication type: Article

Publication status: Published

Journal: Journal of Molecular Neuroscience

Year: 2002

Volume: 18

Issue: 3

Pages: 211-222

ISSN (print): 0895-8696

ISSN (electronic): 1559-1166

Publisher: Humana Press, Inc.

URL: http://dx.doi.org/10.1385/JMN:18:3:211

DOI: 10.1385/JMN:18:3:211

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