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Mechanical stretch regulates cell survival in human bladder smooth muscle cells in vitro

Lookup NU author(s): Professor James Gillespie


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Our understanding of the pathophysiology of the overactive bladder is poor. It has been proposed that localized contractions result in the abnormal stretching of bladder smooth muscle. We hypothesize that stretch regulates the cellular processes that determine tissue size. The purpose of this study was to investigate the effect of stretch on apoptosis, proliferation, cell hypertrophy, and growth factor production in human bladder smooth muscle cells in vitro. Normal human detrusor muscle was obtained from patients undergoing radical cystectomy for invasive bladder cancer, and primary cultures were established. Cells were mechanically stretched on flexible plates at a range of pressures and times. Apoptosis was assessed by propidium iodide incorporation and flow cytometry. Radiolabeled thymidine and amino acid incorporation were used to assess proliferation and cell hypertrophy. ELISA and RT-PCR were used to assess growth factor production. Mechanical stretch inhibits apoptosis in a time- and dose-dependent manner and was associated with increases in the antiapoptotic proteins heat shock protein-70 and cIAP-1. Stretch also increases smooth muscle cell proliferation and hypertrophy, but hypertrophy is the more dominant response. These changes were associated with increases in IGF-1 and basic FGF and a decrease in transforming growth factor-beta1. Mechanical stretch regulates apoptosis, proliferation, and cell hypertrophy in human bladder smooth muscle cells.

Publication metadata

Author(s): Galvin DJ, Watson RWG, Gillespie JI, Brady H, Fitzpatrick JM

Publication type: Article

Publication status: Published

Journal: American Journal of Physiology: Renal Physiology

Year: 2002

Volume: 283

Issue: 6

Pages: F1192-F1199

ISSN (print): 1931-857X

ISSN (electronic): 1522-1466

Publisher: American Physiological Society


DOI: 10.1152/ajprenal.00168.2002


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