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Evaluation of S-aureus ID, a new chromogenic agar medium for detection of Staphylococcus aureus

Lookup NU author(s): Dr John Perry, Professor Kate Gould

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Abstract

S. aureus ID (bioMerieux, La Balme Les Grottes, France) is a new chromogenic agar medium designed to enable the isolation of staphylococci and the specific identification of Staphylococcus aureus. S. aureus produces green colonies on this medium due to production of a-glucosidase. To evaluate this medium, a total of 350 wound swabs were cultured onto S. aureus ID, CHROMagar Staph. aureus, and conventional media routinely used in our laboratory. After 18 to 20 It of incubation, 96.8% of strains formed green colonies on S. aureus ID compared with 91.1% of strains forming mauve colonies on CHROMagar Staph. aureus. A total of 94.3% of strains were recovered within 18 to 20 h with conventional media. The sensitivity was increased after 48 h of incubation to 98.7, 96.2, and 95.6% with S. aureus ID, CHROMagar Staph. aureus, and conventional media, respectively. A total of 97.4% of green colonies on S. aureus ID were confirmed as S. aureus compared with 94.4% of mauve colonies on CHROMagar Staph. aureus. We conclude that S. aureus ID is a highly sensitive and specific medium for the isolation and identification of S. aureus from wound swabs.

Publication metadata

Author(s): Perry JD, Rennison C, Butterworth LA, Hopley ALJ, Gould FK

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Microbiology

Year: 2003

Volume: 41

Issue: 12

Pages: 5695-5698

ISSN (print): 0095-1137

ISSN (electronic): 1098-660X

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/JCM.41.12.5695-5698.2003

DOI: 10.1128/JCM.41.12.5695-5698.2003

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