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Renal epithelium: reversal of cytotoxic damage by addition of anti-thymocyte globulin

Lookup NU author(s): Emeritus Professor John Kirby, Dr Brian Shenton, George Proud, Professor Thomas Lennard, Robert Taylor


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A novel in vitro assay of renal epithelium tight junction function was used to assess the efficacy with which rabbit anti-thymocyte globulin (ATG) blocks epithelium damage mediated by lymphokine-activated killer (LAK) cells. It was found that LAK cells lysed renal epithelial cells poorly in standard chromium-release assays but that they caused a rapid, and almost total, reduction in trans-epithelium monolayer resistance, indicating tight junction failure and, hence, loss of tissue function. LAK cell-mediated cytolysis of the sensitive K562 cell line was completely blocked in the presence of ATG at a concentration of 200-mu-g/ml. Addition of ATG at this concentration to damaged renal cell monolayers in the presence of LAK cells allowed the trans-monolayer resistance to recover rapidly to levels approaching the values recorded before initial addition of LAK cells. On this basis it seems likely that the rapid restoration of renal function frequently observed after appropriate "rescue" therapy during episodes of acute rejection may reflect subtle changes in tissue function rather than recovery from widespread graft cell cytolysis.

Publication metadata

Author(s): Clark KR, Kirby JA, Baker N, Givan AL, Shenton BK, Proud G, Lennard TWJ, Taylor RMR

Publication type: Article

Publication status: Unknown

Journal: Transplant International

Year: 1991

Volume: 4

Issue: 4

Pages: 210-214

Print publication date: 01/01/1991

ISSN (print): 0934-0874

ISSN (electronic): 1432-2277