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Mitogenic activity of procathepsin-D purified from conditioned medium of breast cancer cells by affinity chromatography on pepstatinyl agarose

Lookup NU author(s): Dr Felicity May, Professor Bruce Westley


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A simple procedure for the affinity purification of procathepsin D from tissue culture medium conditioned by breast-cancer cells is described. This procedure yielded 2 mu g of procathepsin D/100 ml medium. The procathepsin D was approximately 95% pure as judged by silver staining of polyacrylamide gels, the major contaminant being mature cathepsin D. The ability of procapthesin D to stimulate the proliferation of oestrogen-responsive MCF-7 breast cancer cells was determined. The purified procapthesin D had no mitogenic effect alone or in combination with oestradiol or other growth factors. These data suggest that procapthesin D does not act as an oestrogen-regulated autocrine growth factor for malignant breast epithelial calls. (C) 1994 Wiley-Liss, Inc.

Publication metadata

Author(s): Stewart AJ, Piggott NH, May FEB, Westley BR

Publication type: Article

Publication status: Published

Journal: International Journal of Cancer

Year: 1994

Volume: 57

Issue: 5

Pages: 715-718

Print publication date: 01/06/1994

ISSN (print): 0020-7136

ISSN (electronic): 1097-0215


DOI: 10.1002/ijc.2910570518


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