Mitogenic activity of procathepsin-D purified from conditioned medium of breast cancer cells by affinity chromatography on pepstatinyl agarose
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Lookup NU author(s): Dr Felicity May,
Professor Bruce Westley
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A simple procedure for the affinity purification of procathepsin D from tissue culture medium conditioned by breast-cancer cells is described. This procedure yielded 2 mu g of procathepsin D/100 ml medium. The procathepsin D was approximately 95% pure as judged by silver staining of polyacrylamide gels, the major contaminant being mature cathepsin D. The ability of procapthesin D to stimulate the proliferation of oestrogen-responsive MCF-7 breast cancer cells was determined. The purified procapthesin D had no mitogenic effect alone or in combination with oestradiol or other growth factors. These data suggest that procapthesin D does not act as an oestrogen-regulated autocrine growth factor for malignant breast epithelial calls. (C) 1994 Wiley-Liss, Inc.
Author(s): Stewart AJ, Piggott NH, May FEB, Westley BR
Publication type: Article
Publication status: Published
Journal: International Journal of Cancer
Print publication date: 01/06/1994
ISSN (print): 0020-7136
ISSN (electronic): 1097-0215
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