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Regulation of glycogen synthesis by amino acids in cultured human muscle cells

Lookup NU author(s): Dr Jane Renwick, Dr Sylvie Bonavaud, Emeritus Professor Steve Yeaman


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Insulin and a number of metabolic factors stimulate glycogen synthesis and the enzyme glycogen synthase. Using human muscle cells we find that glycogen synthesis is stimulated by treatment of the cells with lithium ions, which inhibit glycogen synthase kinase 3. Insulin further stimulates glycogen synthesis in the presence of lithium ions, an effect abolished by wortmannin and rapamycin. We report also that amino acids stimulate glycogen synthesis and glycogen synthase, these effects also being blocked by rapamycin and wortmannin. Amino acids stimulate p70(s6k) and transiently inhibit glycogen synthase kinase 3 without effects on the activity of protein kinase B or the mitogen-activated protein kinase pathway. Thus, the work reported here demonstrates that amino acid availability can regulate glycogen synthesis. Furthermore, it demonstrates that glycogen synthase kinase 3 can be inactivated within cells independent of activation of protein kinase B and p90(rsk).

Publication metadata

Author(s): Armstrong JL, Bonavaud SM, Toole BJ, Yeaman SJ

Publication type: Article

Publication status: Published

Journal: Journal of Biological Chemistry

Year: 2001

Volume: 276

Issue: 2

Pages: 952-956

ISSN (print): 0021-9258

ISSN (electronic): 1083-351X


DOI: 10.1074/jbc.M004812200

PubMed id: 11013237


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