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Determination of estrogen responsiveness of breast cancer by competitive reverse transcription polymerase chain reaction

Lookup NU author(s): Dr Mary Carr, Dr Felicity May, Professor Thomas Lennard, Professor Bruce Westley


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Competitive polymerase chain reaction assays have been developed for the quantitation of oestrogen receptor mRNA and two oestrogen-regulated mRNAs (progesterone receptor and pNR-2/pS2) in breast cancer cells. These assays are more sensitive than traditional hybridisation techniques, do not require the use of radioisotopes, measure absolute amounts of messenger RNAs and can be used to measure the expression of mRNAs in small numbers of tumour cells obtained by fine-needle aspiration (FNA). These assays should prove useful for predicting the hormone responsiveness of breast cancer from tumour cells obtained by FNA at diagnosis and could be particularly useful in the management of elderly/frail patients who receive primary tamoxifen, or in other patients for whom tumour tissue for standard biochemical measurements is not available.

Publication metadata

Author(s): Carr M, May FEB, Lennard TWJ, Westley BR

Publication type: Article

Publication status: Published

Journal: British Journal of Cancer

Year: 1995

Volume: 72

Issue: 6

Pages: 1427-1434

Print publication date: 01/12/1995

ISSN (print): 0007-0920

ISSN (electronic): 1532-1827