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Antipeptide antibodies against the PNR-2-estrogen regulated protein of human breast cancer cells and detection of PNR-2 expression in norman tissue by immunohistochemistry

Lookup NU author(s): Dr Felicity May, Professor Bruce Westley


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Five peptides, corresponding to regions of the predicted protein sequence of the oestrogen-regulated pNR-2 protein which is expressed in oestrogen-responsive human breast cancer cells, were synthesized. Two peptides were immunogenic in rabbits and antisera against one peptide reacted with the pNR-2 protein in sections of formalin-fixed, paraffin-embedded breast tumour. There was a significant correlation between the extent of pNR-2 protein expression detected by immunohistochemistry and pNR-2 mRNA levels determined by hybridization with a cDNA probe in a series of primary breast tumours. pNR-2 expression was assessed immunohistochemically in a panel of normal tissues. Expression was detected in normal breast, small intestine, and stomach (body and antrum).

Publication metadata

Author(s): Piggott NH, Henry JA, May FEB, Westley BR

Publication type: Article

Publication status: Published

Journal: Journal of Pathology

Year: 1991

Volume: 163

Issue: 2

Pages: 95-104

Print publication date: 01/02/1991

ISSN (print): 0022-3417

ISSN (electronic): 1096-9896