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Region 1.2 of the RNA polymerase sigma subunit controls recognition of the -10 promoter element

Lookup NU author(s): Professor Nikolay ZenkinORCiD, Dr Yulia Yuzenkova

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Abstract

Recognition of the -10 promoter consensus element by region 2 of the bacterial RNA polymerase subunit is a key step in transcription initiation. also functions as an elongation factor, inducing transcription pausing by interacting with transcribed DNA non-template strand sequences that are similar to the -10 element sequence. Here, we show that the region 1.2 of Escherichia coli 70, whose function was heretofore unknown, is strictly required for efficient recognition of the non-template strand of -10-like pause-inducing DNA sequence by region 2, and for -dependent promoter-proximal pausing. Recognition of the fork-junction promoter DNA by RNA polymerase holoenzyme also requires region 1.2 and thus resembles the pause-inducing sequence recognition. Our results, together with available structural data, support a model where region 1.2 acts as a core RNA polymerase-dependent allosteric switch that modulates non-template DNA strand recognition by region 2 during transcription initiation and elongation.

Publication metadata

Author(s): Zenkin N, Kulbachinskiy A, Yuzenkova Y, Mustaev A, Bass I, Severinov K, Brodolin K

Publication type: Article

Publication status: Published

Journal: EMBO Journal

Year: 2007

Volume: 26

Issue: 4

Pages: 955-964

ISSN (print): 0261-4189

ISSN (electronic): 1460-2075

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/sj.emboj.7601555

DOI: 10.1038/sj.emboj.7601555

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