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Dietary regulation of ruminal bovine UT-B urea transporter expression and localization

Lookup NU author(s): Professor Nicholas Simmons, Dr Abdul ChaudhryORCiD, Dr Christopher Graham, Dr Gavin Stewart

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Abstract

Facilitative UT-B urea transporters have been located in the gastrointestinal tract of numerous mammalian species. We have previously identified UT-B urea transporters within the epithelial layers of the bovine (b) rumen. The aim of this study was to test the hypothesis that ruminal bUT-B urea transporters are regulated by dietary intake. Six Limousine-cross steers (initial BW = 690 +/- 51 kg) were separated into 2 groups fed a basic silage-based diet (RS) or a concentrate-based diet (RC) for 37 d and compared for ruminal morphology, content, and bUT-B expression. Analysis by reverse transcription-PCR showed that ruminal bUT-B2 mRNA expression was greater in RC-fed than RS-fed animals. Utilizing an anti-bUT-B antibody, we also detected a significant increase in bUT-B2 protein expression in RC-fed rumen (P

Publication metadata

Author(s): Simmons NL, Chaudhry AS, Graham C, Scriven ES, Thistlethwaite A, Smith CP, Stewart GS

Publication type: Article

Publication status: Published

Journal: Journal of Animal Science

Year: 2009

Volume: 87

Issue: 10

Pages: 3288-3299

ISSN (print): 0021-8812

ISSN (electronic): 1525-3163

Publisher: American Society of Animal Science

URL: http://dx.doi.org/10.2527/jas.2008-1710

DOI: 10.2527/jas.2008-1710

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