Browse by author
Lookup NU author(s): Dr Maria Ledran, Professor Lyle Armstrong, Ian Dimmick, Dr Rebecca Lang, Sun Yung, Dr Mauro Santibanez Koref, Professor Miodrag Stojkovic, Professor Majlinda LakoORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Hematopoietic stem cells derived from human embryonic stem cells (hESCs) could provide a therapeutic alternative to bone marrow transplants, but the efficiency of currently available derivation protocols is low. In this study, we investigated whether coculture with monolayers; of cells derived from mouse AGM and fetal liver, or with stromal cell lines derived from these tissues, can enhance hESC hematopoietic differentiation. We found that under such conditions hESC-derived differentiating cells formed early hematopoietic progenitors, with a peak at day 18-21 of differentiation that corresponded to the highest CD34 expression. These hESC-derived hematopoietic cells were capable of primary and secondary hematopoietic engraftment into immunocompromised mice at substantially higher levels than described previously. Transcriptional and functional analysis identified TGF-beta 1 and TGF-beta 3 as positive enhancers of hESC hematopoietic differentiation that can further stimulate this process when added to the culture. Overall, our findings represent significant progress toward the goal of deriving functional hematopoietic stem cells from hESCs.
Author(s): Ledran MH, Krassowska A, Armstrong L, Dimmick I, Renstrom J, Lang R, Yung S, Santibanez-Coref M, Dzierzak E, Stojkovic M, Oostendorp RAJ, Forrester L, Lako M
Publication type: Article
Publication status: Published
Journal: Cell Stem Cell
Year: 2008
Volume: 3
Issue: 1
Pages: 85-98
ISSN (electronic): 1934-5909
Publisher: Cell Press
URL: http://dx.doi.org/10.1016/j.stem.2008.06.001
DOI: 10.1016/j.stem.2008.06.001
Altmetrics provided by Altmetric
