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Lookup NU author(s): Dr Kenneth McKeegan, Charles Debieux, Dr Nick Watkins
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The box C/D small nucleolar RNPs (snoRNPs) are essential for the processing and modification of rRNA. TIP48 and TIP49 are two related AAA(+) proteins that are essential for the formation of box C/D snoRNPs. These proteins are key components of the pre-snoRNP complexes, but their exact role in box C/D snoRNP biogenesis is largely uncharacterized. Here we report that TIP48 and TIP49 interact with one another in vitro, and only the TIP48/TIP49 complex, but not the individual proteins, possesses significant ATPase activity. Loss of TIP48 and TIP49 results in a change in pre-snoRNA levels and a loss of U3 snoRNA signal in the Cajal body. We show that TIP48 and TIP49 make multiple interactions with core snoRNP proteins and biogenesis factors and that these interactions are often regulated by the presence of ATP. Furthermore, we demonstrate that TIP48 and TIP49 efficiently bridge interactions between the core box C/D proteins NOP56 or NOP58 and 15.5K. Our data imply that the snoRNP assembly factor NUFIP can regulate the interactions between TIP48 and TIP49 and the core box C/D proteins. We suggest that snoRNP assembly involves an intricate series of interactions that are mediated/regulated by bridging factors and chaperones.
Author(s): McKeegan KS, Debieux CM, Watkins NJ
Publication type: Article
Publication status: Published
Journal: Molecular and Cellular Biology
Year: 2009
Volume: 29
Issue: 18
Pages: 4971-4981
ISSN (print): 0270-7306
ISSN (electronic): 1098-5549
Publisher: American Society for Microbiology
URL: http://dx.doi.org/10.1128/MCB.00752-09
DOI: 10.1128/MCB.00752-09
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