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FISH (Fluoresecence in situ Hybridization) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia

Lookup NU author(s): Professor Christine Harrison FRCPath FMedSci


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Cytogenetics is integral to the diagnosis of childhood leukaemia, particularly in relation to the risk stratification of patients for treatment. Fluorescence in situ hybridization (FISH) has become an important complementary technique, expanding chromosomal analysis into the molecular arena. It has greatly improved the accuracy and applicability of cytogenetics and led to the discovery of novel chromosomal changes of prognostic significance. Many probes are now commercially available, providing robust and reliable detection of chromosomal abnormalities. Since the cloning of the human genome, it is now possible to access detailed genomic information and develop FISH probes for virtually any known DNA sequence. The range of procedures necessary for the successful application of FISH in the accurate detection of significant chromosomal abnormalities in childhood acute leukaemia is described here.

Publication metadata

Author(s): Konn Z, Wright S, Barber K, Harrison CJ

Editor(s): So, CWE

Publication type: Book Chapter

Publication status: Published

Book Title: Leukemia: Methods and Protocols

Year: 2009

Volume: 538

Pages: 29-55

Edition: 1st

Series Title: Methods in Molecular Medicine

Publisher: Humana Press Inc.

Place Published: New Jersey, USA


DOI: 10.1007/978-1-59745-418-6_3

Notes: Online ISBN: 9781597454186

Library holdings: Search Newcastle University Library for this item

ISBN: 9781588299895