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Cardiac Allograft Rejection: Examination of the Expression and Function of the Decoy Chemokine Receptor D6

Lookup NU author(s): Dr Helen Marshall, Dr Helen Robertson, Professor John Kirby, Professor Simi Ali, Dr Graeme O'Boyle

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Abstract

Background. Inflammatory cell recruitment during allograft rejection is driven by a group of inflammatory cytokines termed chemokines. Chemokines are presented on the surface of the vascular endothelium where they ligate specific receptors expressed on the surface of leukocytes. Recently, a group of nonsignaling chemokine receptors have been described. These bind and internalize chemokines but do not drive leukocyte migration. It is believed that these compete with classical signaling receptors to modulate inflammation. Methods. This study describes the first examination of the human decoy chemokine receptor D6 during rejection; D6 binds at least 12 potent proinflammatory chemokines. The expression of D6 by graft infiltrating leukocytes was examined in cardiac allografts by confocal microscopy on biopsy sections (n = 19). Cytokine regulation of D6 was examined in vitro, and a chemokine scavenging assay was performed using the prototypical transplant-associated chemokine CCL5/RANTES. Results. D6 expression was found to be higher in the biopsies taken from more severe cardiac allograft rejection (P < 0.01) and was predominantly localized to graft infiltrating CD45(+)CD68(+) leukocytes. In vitro studies demonstrated that the transforming growth factor-beta strongly increased the expression of D6 by monocytes, which significantly enhanced D6-mediated chemokine scavenging (by 85%, P < 0.05). Conclusions. We present the first examination of the biology of D6 during rejection and identify a transplant-associated cytokine that is able to regulate its expression. These data suggest an exciting new mechanism for the antiinflammatory actions of transforming growth factor-beta. Understanding the expression patterns of D6 may provide important insight into the regulation and control of inflammatory cell recruitment during allograft rejection.


Publication metadata

Author(s): Bradford L, Marshall H, Robertson H, Kirby JA, Graham G, Ali S, O'Boyle G

Publication type: Article

Publication status: Published

Journal: Transplantation

Year: 2010

Volume: 89

Issue: 11

Pages: 1411-1416

Print publication date: 01/06/2010

ISSN (print): 0041-1337

ISSN (electronic): 1534-6080

Publisher: Lippincott Williams & Wilkins

URL: http://dx.doi.org/10.1097/TP.0b013e3181da604b

DOI: 10.1097/TP.0b013e3181da604b


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Funding

Funder referenceFunder name
British Heart Foundation
078892Wellcome Trust
884289934Roche Organ Transplantation Research Fund

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