Browse by author
Lookup NU author(s): Dr Zoltan Rakonczay, Dr Michael Gray, Professor Barry Argent
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
SLC26 anion exchangers (probably SLC26A3 and SLC26A6) are expressed on the apical membrane of pancreatic duct cells and play a key role in HCO 3- secretion; a process that is inhibited by the neuropeptide, substance P (SP). SP had no effect on basolateral HCO 3- transporters in the duct cell or on CFTR Cl- channels, but inhibited a Cl--dependent HCO3- efflux step on the apical membrane. In microperfused ducts, luminal H 2DIDS (0.5 mM) caused intracellular pH to alkalinize (consistent with inhibition of HCO3- efflux) and, like SP, inhibited HCO3- secretion. SP did not reduce HCO3- secretion further when H2DIDS was applied to the duct lumen, suggesting that SP and H2DIDS inhibit the same transporter on the apical membrane. As SLC26A6 is DIDS-sensitive, this isoform is the likely target for SP. The inhibitory effect of SP was mimicked by phorbol 12,13-dibutyrate (PDBu), an activator of protein kinase C (PKC). Moreover, bisindolylmaleimide, a blocker of PKC, relieved the inhibitory effect of both SP and PDBu on HCO3- secretion. Western blot analysis revealed that guinea pig pancreatic ducts express the α, β1, δ, ε, η, θ, ζ and μ isoforms of PKC. We conclude that PKC is a negative regulator of SLC26 activity in pancreatic duct cells. Copyright © Novartis Foundation 2006.
Author(s): Hegyi P, Rakonczay Jr Z, Tiszlavicz L, Varro A, Toth A, Racz G, Varga G, Gray MA, Argent BE
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: Epithelial Anion Transport: The Role of the SLC26 Transporters Family
Year of Conference: 2006
Pages: 164-173
Publisher: John Wiley & Sons
PubMed id: 17120767
Library holdings: Search Newcastle University Library for this item
Series Title: Novartis Foundation Symposium
ISBN: 9780470016244
