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Treatment of the brain-dead lung donor with aprotinin and nitric oxide

Lookup NU author(s): Vassillios Avlonitis, Christopher Wigfield, Emeritus Professor John Kirby, Professor John Dark


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BACKGROUND: It has been previously shown that donor treatment with aprotinin or inhaled nitric oxide reduces reperfusion injury after lung transplantation in animals. These studies used living donors with normal lungs. However, the main source of lungs for transplantation is brain-dead donors. Brain death causes systemic inflammatory response and lung injury, rendering the organ susceptible to reperfusion injury after transplantation. We hypothesized that treatment with aprotinin or inhaled nitric oxide after brain death would improve the donor inflammatory response and reduce lung reperfusion injury after transplantation. METHODS: Brain death was induced in 24 rats by intracranial balloon inflation. Subsequently, the animals received intravenous aprotinin (n = 8), inhaled nitric oxide (n = 7), or no treatment (n = 9) for 5 hours. The lungs were retrieved and reperfused for 2 hours using recipient rats. RESULTS: After brain death, oxygenation deteriorated earlier and significantly more in rats that received treatment, especially with nitric oxide. Treatment did not reduce the donor systemic inflammatory response as assessed by serum levels of proinflammatory cytokines. Oxygenation, airway pressure, pulmonary vascular resistance, lung water index and bronchoalveolar lavage cytokine levels were similar after reperfusion of grafts from all three groups of donors. CONCLUSIONS: Donor treatment with aprotinin or inhaled nitric oxide does not improve lungs that have been injured by brain death. J Heart Lung Transplant 2010;29:1177-84 (C) 2010 International Society for Heart and Lung Transplantation. All rights reserved.

Publication metadata

Author(s): Avlonitis VS, Wigfield CH, Kirby JA, Dark JH

Publication type: Article

Publication status: Published

Journal: Journal of Heart and Lung Transplantation

Year: 2010

Volume: 29

Issue: 10

Pages: 1177-1184

Print publication date: 01/10/2010

ISSN (print): 1053-2498

ISSN (electronic): 1557-3117

Publisher: Elsevier Inc.


DOI: 10.1016/j.healun.2010.05.024


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Funder referenceFunder name
066501Wellcome Trust