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Characterization of a GSK-3 inhibitor in culture of human cord blood primitive hematopoietic cells

Lookup NU author(s): Dr Xiao WangORCiD

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Abstract

Wnt signaling pathway plays important roles in the biology of stem cells in maintaining their self-renewal property. Glycogen synthase kinase-3 (GSK-3) inhibitors, the Wnt signaling agonists, maintain the pluripotency of embryonic stem cells. We report here that a synthetic GSK-3 inhibitor, 6-bromoindirubin-3'-oxime (BIO), showed opposite effects on the expansion of human primitive hematopoietic cells isolated from umbilical cord blood (UCB). In combination with human c-kit ligand (KL), BIO at low concentration (0.2 mu M) enhanced the expansion of UCB CD34(+) cells, which was BIO structure and exposure time dependent; however, at high concentration (2 mu M) it inhibited the expansion of the cells. Furthermore, hematopoietic stem cells (HSCs) were exhausted when the UCB CD34(+) cells were exposed to 0.2 mu M BIO and KL longer than 2 days. In conclusion, the use of BIO in expansion of UCB HSCs remains a significant challenge. (C) 2010 Elsevier Masson SAS. All rights reserved.

Publication metadata

Author(s): Jiang JF, Zhao M, Zhang AY, Yu MF, Lin XJ, Wu MY, Wang XN, Lu HL, Zhu SY, Yu Y, Mao ZM, Han W

Publication type: Article

Publication status: Published

Journal: Biomedicine & Pharmacotherapy

Year: 2010

Volume: 64

Issue: 7

Pages: 482-486

Print publication date: 04/03/2010

ISSN (print): 0753-3322

ISSN (electronic): 1950-6007

Publisher: Elsevier Masson

URL: http://dx.doi.org/10.1016/j.biopha.2010.01.005

DOI: 10.1016/j.biopha.2010.01.005

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